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Luminescence

Current research reports and chronological list of recent articles..




Luminescence - The Journal of Biological and Chemical Luminescence - provides a forum for the publication of original scientific papers, short communications, technical notes and reviews on fundamental and applied aspects of all forms of luminescence, including bioluminescence, chemiluminescence, electrochemiluminescence, sonoluminescence, triboluminescence, fluorescence, time-resolved fluorescence and phosphorescence.

The publisher is Wiley. The copyright and publishing rights of specialized products listed below are in this publishing house. This is also responsible for the content shown.

To search this web page for specific words type "Ctrl" + "F" on your keyboard (Command + "F" on a Mac). Then: type the word you are searching for in the window that pops up!

Additional research articles see Current Chemistry Research Articles. General information about this topic see bioluminescence.



Luminescence - Abstracts



Bioluminescence-based cytotoxicity assay for simultaneous evaluation of cell viability and membrane damage in human hepatoma HepG2 cells

We have developed a bioluminescence-based non-destructive cytotoxicity assay in which cell viability and membrane damage are simultaneously evaluated using Emerald luciferase (ELuc) and endoplasmic reticulum (ER)-targeted copepod luciferase (GLuc-KDEL), respectively, by using multi-integrase mouse artificial chromosome (MI-MAC) vector. We have demonstrated that the time-dependent concentration response curves of ELuc luminescence intensity and WST-1 assay, and GLuc-KDEL luminescence intensity and lactate dehydrogenase (LDH) activity in the culture medium accompanied by cytotoxicity show good agreement in toxicant-treated ELuc- and GLuc-KDEL-expressing HepG2 stable cell lines. We have clarified that the increase of GLuc-KDEL luminescence intensity in the culture medium reflects the type of cell death, including necrosis and late apoptosis, but not early apoptosis. We have also uncovered a strong correlation between GLuc-KDEL luminescence intensity in the culture medium and the extracellular release of high mobility group box 1 (HMGB1), a representative damage-associated molecular pattern (DAMP) molecule. The bioluminescence measurement assay using ELuc and GLuc-KDEL developed in this study can simultaneously monitor cell viability and membrane damage, respectively, and the increase of GLuc-KDEL luminescence intensity in the culture medium accompanied by the increase of cytotoxicity is an index of necrosis and late apoptosis associated with the extracellular release of DAMP molecules.
Datum: 09.02.2018


Luminescent properties of novel red-emitting M7Sn(PO4)6:Eu3+ (M = Sr, Ba) for light-emitting diodes

Novel red-emitting phosphors, Eu3+-activated M7Sn(PO4)6 (M = Sr, Ba), were synthesized at 1200°C by conventional solid-state reaction method. The luminescent properties of M7Sn(PO4)6:Eu3+ (M = Sr, Ba) phosphors were investigated, and the critical concentration of the activator (Eu3+) concentration were found to be 0.175 mol and 0.21 mol per formula unit for Sr7-xSn(PO4)6:xEu3+ and Ba7-xSn(PO4)6:xEu3+, respectively. These phosphors presented red luminescence under the excitation of 395 or 465 nm, perfectly matching with the emissions wavelength of near-ultraviolet (UV) light-emitting diodes (LEDs) and InGaN blue LED.
Datum: 06.02.2018


A red-emitting indolium fluorescence probe for membranes - flavonoids interactions

The red-emitting indolium derivative compound (E)-2-(4-(diphenylamino)styryl)-1,3,3-trimethyl-3H-indol-1-ium iodide (H3) was demonstrated as a sensitive membrane fluorescence probe. The probe located at the interface of liposomes when mixed showed much fluorescence enhancement by inhibiting the twisted intramolecular charge transfer state. After ultrasonic treatment, it penetrated into lipid bilayers with the emissions leveling off and a rather large encapsulation efficiency (71.4%) in liposomes. The ζ-potential and particle size measurement confirmed that the charged indolium group was embedded deeply into lipid bilayers. The probe was then used to monitor the affinities of antioxidant flavonoids for membranes. It was verified that quercetin easily interacted with liposomes and dissociated the probe from the internal lipid within 60 s under the condition of simply mixing. The assessment of binding affinities of six flavonoids and the coincident results with their antioxidation activities indicated that it was a promising membrane probe for the study of drug bio-affinities.
Datum: 06.02.2018


Tunable excitation properties of ZnCdS:Mn/ZnS quantum dots for cancer imaging

Water-soluble ZnS:Mn quantum dots (QDs) were synthesized using a hydrothermal method with 3-mercaptopropionic acid as stabilizer. The optical properties of ZnS:Mn QDs were thoroughly investigated by tuning the doping concentration of Mn2+ and the Zn/S precursor ratio, to obtain an optimal parameter for QDs with excellent fluorescence characteristics. ZnS:Mn QDs excited at only one wavelength, however, which seriously limited their further application. Here, a trace Cd ion was doped into a ZnS host, resulting in QD excitation covering a wide adjustable waveband. Furthermore, when a ZnS shell was coated onto the surface of the ZnCdS:Mn QDs, photoluminescence intensity and stability were further enhanced. After coupling with an anti-CK 19 antibody, the ZnCdS:Mn/ZnS core/shell QDs were able to function by labeling cancer cells, indicating that they could be considered as a suitable bio-probe for cells and tissue imaging.
Datum: 05.02.2018


The fluorescence quenching of Ru(bipy)32+: an application for the determination of bilirubin in biological samples

A simple, sensitive and efficient fluorescence method has been established for the quantitative analysis of bilirubin. The fluorometric determination method was based on the kinetic quenching of ruthenium(II) fluorescence. The quenching effect may be due to the complexation reaction of bilirubin with ruthenium(II). Therefore, the effects of ruthenium concentrations and different surfactants have been studied. Under the optimized experimental parameters, the fluorescence intensity decreased proportionally with the bilirubin concentration and linearity was established in the range of 3.3 × 10−7 to 3.0 × 10−4 M bilirubin. The detection limit calculated from the calibration graph was found to be 5.2 × 10−8 M. The relative standard deviation (RSD) of 10 consecutive measurements of 8.0 × 10−6 M bilirubin was 3.0%, while the recoveries of bilirubin in both human serum and urine samples were obtained in the range 94.0–99.5%. The interference study shows that the developed fluorescence based technique is fast, easy to carry out and shows negligible interference. The developed technique was successfully applied for the analysis of bilirubin in human urine and serum samples. All the experimental results and quality parameters confirmed the sensitivity and reproducibility of the proposed technique for bilirubin determination in human urine and serum samples.
Datum: 05.02.2018


A fluorescent aptasensor for analysis of adenosine triphosphate based on aptamer–magnetic nanoparticles and its single-stranded complementary DNA labeled carbon dots

A new fluorimetric aptasensor was designed for the determination of adenosine triphosphate (ATP) based on magnetic nanoparticles (MNPs) and carbon dots (CDs). In this analytical strategy, an ATP aptamer was conjugated on MNPs and a complementary strand of the aptamer (CS) was labeled with CDs. The aptamer and its CS were hybridized to form a double helical structure. The hybridized aptamers could be used for the specific recognition of ATP in a biological complex matrix using a strong magnetic field to remove the interfering effect. In the absence of ATP, no CDs–CS could be released into the solution and this resulted in a weak fluorescence signal. In the presence of ATP, the target binds to its aptamer and causes the dissociation of the double helical structure and liberation of the CS, such that a strong fluorescence signal was generated. The increased fluorescence signal was proportional to ATP concentration. The limit of detection was estimated to be 1.0 pmol L–1 with a dynamic range of 3.0 pmol L–1 to 5.0 nmol L–1. The specific aptasensor was applied to detect ATP in human serum samples with satisfactory results. Moreover, molecular dynamic simulation (MDS) studies were used to analyze interactions of the ATP molecule with the aptamer.
Datum: 30.01.2018


Luminescent nanoparticles for rapid monitoring of endogenous acetylcholine release in mice atria

The present work introduces for the first time a nanoparticulate approach for ex vivo monitoring of acetylcholinesterase-catalyzed hydrolysis of endogenous acetylcholine released from nerve varicosities in mice atria. Amino-modified 20-nm size silica nanoparticles (SNs) doped by luminescent Tb(III) complexes were applied as the nanosensors. Their sensing capacity results from the decreased intensity of Tb(III)-centred luminescence due to the quenching effect of acetic acid derived from acetylcholinesterase-catalyzed hydrolysis of acetylcholine. Sensitivity of the SNs in monitoring acetylcholine hydrolysis was confirmed by in vitro experiments. Isolated atria were exposed to the nanosensors for 10 min to stain cell membranes. Acetylcholine hydrolysis was monitored optically in the atria samples by measuring quenching of Tb(III)-centred luminescence by acetic acid derived from endogenous acetylcholine due to its acetylcholinesterase-catalyzed hydrolysis. The reliability of the sensing was demonstrated by the quenching effect of exogenous acetylcholine added to the bath solution. Additionally, no luminescence quenching occurred when the atria were pre-treated with the acetylcholinesterase inhibitor paraoxon.
Datum: 29.01.2018


Synthesis and properties of blue luminescent bipolar materials constructed with carbazole and anthracene units with 4-cyanophenyl substitute at the 9-position of the carbazole unit

With carbazole and p-cyanobromobenzene as raw materials, 4-(3,6-di (anthracen-9-yl)-9H-carbazol-9-yl)benzonitrile (DACB) and 4-(3,6-bis(anthracene -9-ylethynyl)-9H-carbazol-9-yl)benzonitrile (BACB) were synthesized through the Suzuki coupling reaction and the Sonogashira coupling reaction, respectively. These structures were characterized using 1H nuclear magnetic resonance (NMR), elemental analysis and mass spectrometry. Their thermal properties, ultraviolet–visible (UV-vis) absorption, fluorescence emission, fluorescence quantum yields and electrochemical properties were also investigated systematically. In addition, a electroluminescence (EL) device was made with BACB as the emitting layer and performance of the EL device was studied. Results showed that: (1) the temperature points with 5% and 10% of DACB weight loss were 443°C and 461°C, respectively, and were 475°C and 506°C with BACB weight loss of 5% and 10%, respectively. When the temperature was 50−300°C, no significantly thermal transition was observed which suggested that they had excellent thermal stability. (2) DACB and BACB had single emission peaks at 415 nm, and 479 nm with fluorescence quantum yields of 0.61 and 0.87, respectively, indicating that both compounds could emit strong blue light. (3) According to electrochemical measurement on BACB and DACB, their gaps were 3.07 eV and 2.76 eV, respectively, which further showed that these two compounds were very stable and acted as efficient blue light materials. (4) The turn-on voltage of the device was 5 V, and the device emitted dark blue light with Commission Internationale de L'Eclairage (CIE) coordinates of (0.157, 0.079).
Datum: 29.01.2018


A new fluorescent chemosensor for cadmium(II) based on a pyrene-appended piperidone derivative and its β-cyclodextrin complex

We report, in this article, a piperidin-4-one derivative carrying pyrenyl fluorescent reporter groups which acts as a Cd2+ ion sensor. The compound is synthesized and characterized using IR and NMR spectral techniques. The compound forms an inclusion complex with β-cyclodextrin. It selectively binds to Cd2+ ions in water and aqueous β-cyclodextrin media. The stoichiometry of the host–guest complex of the compound with β-cyclodextrin is 1:2. The ligand–metal ion binding stoichiometry is 1:1 both in water and in β-cyclodextrin. The linear concentration range of detection of the metal ion is reported. Cyclodextrin complex formation does not affect the metal ion selectivity of the compound.
Datum: 25.01.2018


Luminescent thermometer based on Eu3+/Tb3+-organic-functionalized mesoporous silica

In this work we investigate a mesoporous silica (MS) decorated with dipyridyl-pyridazine (dppz) ligands and further grafted with a mixture of Eu3+/Tb3+ ions (28.45%:71.55%), which was investigated as a potential thermometer in the 10–360 K temperature range. The MS material was prepared employing a hetero Diels–Alder reaction: 3,6-di(2-pyridyl)-1,2,4,5-tetrazine was reacted with the double bonds of vinyl-silica (vSilica) followed by an oxidation procedure. We explore using the dppz-vSilica material to obtain visible emitting luminescent materials and for obtaining a luminescent thermometer when grafted with Eu3+/Tb3+ ions. For the dppz-vSilica@Eu,Tb material absolute sensitivity Sa of 0.011 K−1 (210 K) and relative sensitivity Sr of 1.32 %K−1 (260 K) were calculated showing good sensing capability of the material. Upon temperature change from 10 K to 360 K the emission color of the material changed gradually from yellow to red.
Datum: 25.01.2018


Structural and luminescence properties of Sm3+-doped bismuth phosphate glass for orange-red photonic applications

In the present study, the effect of bismuth oxide (Bi2O3) content on the structural and optical properties of 0.5Sm3+-doped phosphate glass and the effect of concentration on structural and optical properties of Sm3+-doped bismuth phosphate (BiP) glass were studied. Structural characterization was accomplished using X-ray diffraction (XRD), scanning electron microscopy (SEM) with energy dispersive spectroscopy (EDS), Fourier transform infrared (FTIR) spectroscopy and 31P nuclear magnetic resonance (NMR) spectroscopy. Optical properties were studied using absorption, photoluminescence and decay measurements. Using optical absorption spectra, Judd–Ofelt parameters were derived to determine the local structure and bonding in the vicinity of Sm3+ ions. The emission spectra of Sm3+-doped BiP glass showed two intense emission bands, 4G5/26H7/2 (orange) and 4G5/26H9/2 (red) for which the stimulated emission cross-sections (σe) and branching ratios (β) were found to be higher. The quantum efficiencies were also calculated from decay measurements recorded for the 4G5/2 level of Sm3+ ions. The suitable combination of Bi2O3 (10 mol%) and Sm3+ (0.5 mol%) ions in these glasses acted as an efficient lasing material and might be suitable for the development of visible orange-red photonic materials.
Datum: 24.01.2018


Uranyl tris nitrato as a luminescent probe for trace water detection in acetonitrile

Uranyl tris nitrato i.e. [UO2(NO3)3]– was formed by adding tetramethylammonium nitrate to uranyl nitrate in acetonitrile medium. The luminescence features of this complex in acetonitrile are very sensitive to water content, which could lead to the use of it as a luminescent probe for water present in acetonitrile. The luminescence intensity ratio of 507 to 467 nm peak of uranyl tris nitrato showed a linear response in the range 0–5% (v/v) water content in acetonitrile. The present method was applied for three synthetic samples of acetonitrile for water detection and the results obtained were compared using Karl Fischer titration. There was a good agreement in the values obtained by both the methods.
Datum: 22.01.2018


Quenching of graphene quantum dots fluorescence by alkaline phosphatase activity in the presence of hydroquinone diphosphate

In this work, a turn-off photoluminescent sensing proof-of-concept based on blue luminescent graphene quantum dots (GQDs) as the fluorescent probe was developed. For that purpose, GQDs optical response was related with the catalytic enzymatic activity of alkaline phosphatase (ALP), in the presence of hydroquinone diphosphate (HQDP). The hydrolysis of HQDP by ALP generated hydroquinone (HQ). The oxidation of HQ, enzymatically produced, to p-benzoquinone (BQ) resulted in the quenching of GQDs fluorescence (FL). Therefore, the developed luminescent sensing mechanism allowed the FL quenching with ALP activity to be related and thus quantified the concentration of ALP down to 0.5 nM of enzyme. This innovative design principle appears as a promising tool for the development of enzymatic sensors based on ALP labeling with fluorescent detection or even for direct ALP luminescent quantification in an easy, fast and sensitive manner.
Datum: 22.01.2018


Ultrasensitive detection of Shiga toxin 2 and its variants in Shiga toxin-producing Escherichia coli strains by a time-resolved fluorescence immunoassay

A rapid and sensitive two-step time-resolved fluorescence immunoassay (TRFIA) was developed for the detection of Shiga toxin 2 (Stx2) and its variants in Shiga toxin-producing Escherichia coli (STEC) strains. In sandwich mode, a monoclonal antibody against Stx2 was coated on a microtiter plate as a capture antibody. A tracer antibody against Stx2 labeled with europium(III) (Eu3+) chelate was then used as a detector, followed by fluorescence measurements using time-resolved fluorescence. The sensitivity of Stx2 detection was 0.038 ng/ml (dynamic range, 0.1–1000 ng/ml). The intra- and inter-assay coefficients of variation of the assay were 3.2% and 3.6%, respectively. The performance of the established assay was evaluated using culture supernatants of STEC strains, and the results were compared to those of a common HRP (horseradish peroxidase) labeling immunosorbent assay. A polymerase chain reaction (PCR) for the detection of genes encoding Stx1 and Stx2 was used as the reference for comparison. Correlation between the Stx2-specific TRFIA and PCR was calculated by the use of kappa statics, exhibiting a perfect level of agreement. The availability of the sensitive and reliable Stx2-specific TRFIA method for quantifying Stx2 and its variants in STEC strains will complement bacteria isolation-based platform and aid in the accurate and prompt diagnosis of STEC infections.
Datum: 19.01.2018


Synthesis of novel Dy3+ activated Ba2CaZn2Si6O17 phosphors for white light-emitting diodes

Dysprosium ion (Dy3+) activated Ba2CaZn2Si6O17 phosphors were synthesized using high temperature solid-state reaction method. Powder X-ray diffraction (PXRD) analysis confirmed the phase formation of the as-prepared phosphors. Scanning electron microscopy (SEM) analysis disclosed an agglomeration of particles with an irregular morphology. Under 350 nm excitation, the emission spectrum of Dy3+ ions showed bands at 481 nm (blue), 577 nm (yellow) and 674 nm (red). The influence of the Dy3+ concentration on its emission intensity was investigated. The optimum concentration of Dy3+ ions in the Ba2CaZn2Si6O17:Dy3+ phosphors were found to be x = 0.06. The critical energy transfer distance was calculated. The fluorescence lifetime was also determined for Ba2CaZn2Si6O17:0.06Dy3+. The Commission International deI’Eclairage (CIE) chromaticity coordinates of the phosphor were calculated to be x = 0.304, y = 0.382. The activation energy for the thermal quenching was calculated to be 0.168 eV. These results indicated that the Ba2CaZn2Si6O17:Dy3+ phosphor might be a potential candidate for near ultraviolet (NUV)-based white light-emitting diodes.
Datum: 17.01.2018


Synthesis and luminescence properties of cubic-shaped Ca1-xTiO3:Eu3+ particles

In this article Ca1-xTiO3:xEu3+ single crystalline particles with a cubic morphology and average size of 248 to 815 nm were synthesized by a solvothermal method. The structural and optical properties of the Ca1-xTiO3:xEu3+ cubes were investigated, the formation mechanism of the cubes were analyzed and discussed, and the influence of Eu doping content and cubic size on the photoluminescence were examined. The differences in the photoluminescence between Ca1-xTiO3:xEu3+ cubic crystals and nanoparticles was analyzed. It was found that an addition of a small amount of water can substantially reduce the size of the cubes. An obvious red emission band centered at 615 nm was observed under the excitation at 395 nm for the cubes. Our results demonstrate CaTiO3 cubes are good host materials for designing red phosphors.
Datum: 17.01.2018


Behavioural responses of the yellow emitting annelid Tomopteris helgolandica to photic stimuli

In contrast to most mesopelagic bioluminescent organisms specialised in the emission and reception of blue light, the planktonic annelid Tomopteris helgolandica produces yellow light. This unusual feature has long been suggested to serve for intraspecific communication. Yet, this virtually admitted hypothesis has never been tested. In this behavioural study of spectral colour sensitivity, we first present an illustrated repertoire of the postures and action patterns described by captive specimens. Then video tracking and motion analysis are used to quantify the behavioural responses of singled out worms to photic stimuli imitating intraspecific (yellow) or interspecific (blue) bioluminescent signals. We show the ability of T. helgolandica to react and to contrast its responses to bioluminescent-like blue and yellow light signals. In particular, the attractive effect of yellow light and the variation of angular velocity observed according to the pattern of yellow stimuli (flashes versus glows) support the intraspecific communication hypothesis. However, given the behavioural patterns of T. helgolandica, including mechanically induced light emission, the possibility that bioluminescence may be part of escape/defence responses to predation, should remain an open question.
Datum: 16.01.2018


A novel red emitting phosphor LiBaB9O15:Sm2+/Sm3+, li+ with broad excitation band for white LEDs

A novel tunable red emitting phosphor LiBaB9O15:Sm2+/Sm3+, Li+ with broad excitation band was synthesized by a high temperature solid-state method. Luminescence properties were investigated in detail by luminescence, X-ray photoelectron spectroscopy (XPS) spectra and CIE chromaticity coordinates. XPS data confirmed that there were Sm3+ in LiBaB9O15:Sm3+ and Sm2+/Sm3+ in LiBaB9O15:Sm2+/Sm3+, respectively. Spectral property of LiBaB9O15:Sm3+, LiBaB9O15:Sm3+/Sm2+ and LiBaB9O15:Sm2+, Li+ presented that the excitation band of Sm3+ widened and the excitation band of Sm2+ ranged from 350 to 450 nm. And the red light color is tunable with changing Li+ concentration. The results indicated that LiBaB9O15:Sm2+/Sm3+, Li+ may be promising red phosphor for white light emitting diodes.
Datum: 12.01.2018


Solvatochromic study of 3-N-(N′-methylacetamidino)benzanthrone and its interaction with dopamine by the fluorescence quenching mechanism

The change in photophysical properties of the organic molecule due to solvatochromic effect caused by different solvent environments at room temperature gives information about the dipole moments of 3-N-(N′-methylacetamidino)benzanthrone (3-MAB). The quantum yield, fluorescence lifetime of 3-MAB was measured in different solvents to calculate radiative and non-radiative rate constants. The results revealed that the excited state dipole moment (μe) is relatively larger compared to the ground state dipole moment (μg), indicating the excited state of the dye under study is more polar than the ground state and the same trend is noticed with theoretical calculations performed using the CAM-B3LYP/6-311+G(d,p) method. Further, the study on preferential solvation was carried out for 3-MAB dye in ethyl acetate–methanol solvent mixture. The fluorescence quenching method has been employed for the detection of dopamine using 3-MAB as fluorescent probe, using steady-state and time resolved methods at room temperature. The method enables dopamine in the micro molar range to be detected. Also, an attempt to verify the quenching process by employing different models has been tried. Various rate parameters are measured using these models, our results indicates the quenching process is diffusion limited.
Datum: 12.01.2018


Issue Information

No abstract is available for this article.
Datum: 12.01.2018


Luminescence of lemon-derived carbon quantum dot and its potential application in luminescent probe for detection of Mo6+ ions

This article reports on the first attempt of a systematic study on the synthesis of carbon dots (C-dots) for the potential applications in labeling and detection of molybdenum ion (Mo6+). Carbon dots (C-dots) were synthesized directly via a simple hydrothermal method using lemon juices as carbon precursor with different temperatures to control the luminescence of C-dots. The obtained C-dots had strong green light emission and the ability to use its luminescence properties as probes for Mo6+ detection application, which is based on Mo6+ induced luminescence quenching of C-dots. This analysis system exhibits strong sensitivity and good selectivity for Mo6+ ion, and a detection limit as low as 20 ppm is achieved. These results suggest that the present C-dots have potential application in optoelectronic, labeling and luminescent probing of Mo6+ ions.
Datum: 09.01.2018


Effect of CaCN2 on the preparation of CaAlSiN3:Eu2+ phosphors

Highly efficient red-emitting phosphors, CaAlSiN3:Eu2+, were successfully prepared by the solid-state method using calcium cyanide (CaCN2) as the single calcium source. The influences of crystallization temperature, crystallization time, calcination mode and compounds ratio on the photoluminescent properties were investigated. The CaAlSiN3:Eu2+ phosphors were obtained with 1 mol% CaCN2 by a two-step calcination procedure at 900°C for 2 h and subsequently at 1600°C for 8 h. The CaAlSiN3:Eu2+ phosphors showed the strongest luminescent intensity at 660 nm when excited by 468 nm. With an increase in crystallization time, the maximum wavelength of the emission was shifted from 644 nm to 660 nm.
Datum: 09.01.2018


Luminescence properties of Tm3+ ions single-doped YF3 materials in an unconventional excitation region

According to the spectral distribution of solar radiation at the earth's surface, under the excitation region of 1150 to 1350 nm, the up-conversion luminescence of Tm3+ ions was investigated. The emission bands were matched well with the spectral response region of silicon solar cells, achieved by Tm3+ ions single-doped yttrium fluoride (YF3) phosphor, which was different from the conventional Tm3+/Yb3+ ion couple co-doped materials. Additionally, the similar emission bands of Tm3+ ions were achieved under excitation in the ultraviolet region. It is expected that via up-conversion and down-conversion routes, Tm3+-sensitized materials could convert photons to the desired wavelengths in order to reduce the energy loss of silicon solar cells, thereby enhancing the photovoltaic efficiency.
Datum: 09.01.2018


Effect of triazole-tryptophan hybrid on the conformation stability of bovine serum albumin

The effect of a potent antimicrobial compound bearing 1,2,3-triazole core and a tryptophan tail, triazole-tryptophan hybrid (TTH), with bovine serum albumin (BSA) have been explored using various spectroscopic and molecular docking methods. Studies revealed that TTH strongly quenches the intrinsic fluorophore of BSA by a static quenching mechanism. Time-resolved fluorescence spectra further confirmed the involvement of static quenching for TTH–BSA system. The calculated thermodynamic parameters; ΔH, ΔS, and ΔG showed that the binding process was spontaneous, exothermic and entropy driven. Synchronous fluorescence, three-dimensional (3D) fluorescence and circular dichroism data revealed that TTH induces the structural alteration in BSA and enhances its stability. In silico study of TTH–BSA system showed that it binds with BSA at the site I of subdomain IIA. Both the experimental and in silico study showed that the hydrophobic and electrostatic interactions play a major role in TTH–BSA binding.
Datum: 05.01.2018


Full analytical evaluation of the line shift and line width of ions in solids for Raman processes

In this paper, we present a new analytical method to evaluate the temperature dependence of the thermal line shift and thermal line width of spectral lines in the Raman process using a simple approximation for the Debye functions. The proposed formulae guarantee the accurate and fast calculation of the thermal line shift and thermal line width. As an example of application, the analytical expression obtained is used to calculate the line shift and line width of the 2E  4A2 transitions in V2+:MgO at temperatures from 0 K up to 500 K. This analytical evaluation shows that our results are satisfactory for the wide range temperature variations.
Datum: 05.01.2018


Emission analysis of Tb3+-and Sm3+-ion-doped (Li2O/Na2O/K2O) and (Li2O + Na2O/Li2O + K2O/K2O + Na2O)-modified borosilicate glasses

Four series of borosilicate glasses modified by alkali oxides and doped with Tb3+ and Sm3+ ions were prepared using the conventional melt quenching technique, with the chemical composition 74.5B2O3 + 10SiO2 + 5MgO + R + 0.5(Tb2O3/Sm2O3) [where R = 10(Li2O /Na2O/K2O) for series A and C, and R = 5(Li2O + Na2O/Li2O + K2O/K2O + Na2O) for series B and D]. The X-ray diffraction (XRD) patterns of all the prepared glasses indicate their amorphous nature. The spectroscopic properties of the prepared glasses were studied by optical absorption analysis, photoluminescence excitation (PLE) and photoluminescence (PL) analysis. A green emission corresponding to the 5D4 7F5 (543 nm) transition of the Tb3+ ions was registered under excitation at 379 nm for series A and B glasses. The emission spectra of the Sm3+ ions with the series C and D glasses showed strong reddish-orange emission at 600 nm (4G5/2 6H7/2) with an excitation wavelength λexci = 404 nm (6H5/24F7/2). Furthermore, the change in the luminescence intensity with the addition of an alkali oxide and combinations of these alkali oxides to borosilicate glasses doped with Tb3+ and Sm3+ ions was studied to optimize the potential alkali-oxide-modified borosilicate glass.
Datum: 28.12.2017


Comprehensive study of interaction between biocompatible PEG-InP/ZnS QDs and bovine serum albumin

Polyethylene glycol (PEG) surface modified biocompatible InP/ZnS quantum dots (QDs) act as a potential alternative for conventional carcinogenic cadmium-based quantum dots for in vivo and in vitro studies. Comprehensively, we studied the interaction between a model protein bovine serum albumin (BSA) and PEGylated toxic free InP/ZnS QDs using various spectroscopic tools such as absorption, fluorescence quenching, time resolved and synchronous fluorescence spectroscopic measurements. These studies principally show that tryptophan (Trp) residues of BSA have preferable binding affinity towards PEG-InP/ZnS QDs surface and a blue shift in Trp fluorescence emission is a signature of conformational changes in its hydrophobic microenvironment. Photoluminescence (PL) intensity of Trp is quenched by ground state complex formation (static quenching) at room temperature. However, InP/ZnS@BSA conjugates become unstable with increasing temperature and PL intensity of Trp is quenched via dynamic quenching by PEG-InP/ZnS QDs. Experimentally determined thermodynamic parameters for these conjugates have shown spontaneity, entropy driven and exothermic nature of bio-conjugation. The calculated binding affinity (n ≅ 1, Hill coefficient) suggest that the affinity of InP/ZnS QDs for a BSA protein is not dependent on whether or not other BSA proteins are already bound to the QD surface. Energy transfer efficiency (E), Trp residue to InP/ZnS QDs distances and energy transfer rate (kT) were all obtained from FÖrster resonance energy.
Datum: 28.12.2017


Rapid, controllable, one-pot and room-temperature aqueous synthesis of ZnO:Cu nanoparticles by pulsed UV laser and its application for photocatalytic degradation of methyl orange

Zinc oxide (ZnO) and ZnO:Cu nanoparticles (NPs) were synthesized using a rapid, controllable, one-pot and room-temperature pulsed UV-laser assisted method. UV-laser irradiation was used as an effective energy source in order to gain better control over the NPs size and morphology in aqueous media. Parameters effective in laser assisted synthesis of NPs such as irradiation time and laser shot repetition rate were optimized. Photoluminescence (PL) spectra of ZnO NPs showed a broad emission with two trap state peaks located at 442 and 485 nm related to electronic transition from zinc interstitial level (IZn) to zinc vacancy level (VZn) and electronic transition from conduction band to the oxygen vacancy level (VO), respectively. For ZnO:Cu NPs, trap state emissions disappeared completely and a copper (Cu)-related emission appeared. PL intensity of Cu-related emission increased with the increase in concentration of Cu2+, so that for molar ratio of Cu:Zn 2%, optimal value of PL intensity was obtained. The photocatalytic activity of Cu-doped ZnO revealed 50 and 100% increasement than that of undoped NPs under UV and visible irradiation, respectively. The enhanced photocatalytic activity could be attributed to smaller crystal size, as well as creation of impurity acceptor levels (T2) inside the ZnO energy band gap.
Datum: 28.12.2017


High-performance liquid chromatography study of gatifloxacin and sparfloxacin using erythrosine as post-column resonance Rayleigh scattering reagent and mechanism study

Herein, a highly selective high-performance liquid chromatography (HPLC) coupled with resonance Rayleigh scattering (RRS) method was developed to detect gatifloxacin (GFLX) and sparfloxacin (SPLX). GFLX and SPLX were first separated by HPLC, then, in pH 4.4 Britton–Robinson (BR) buffer medium, protonic quaternary ammonia cation of GFLX and SPLX reacted with erythrosine (ERY) to form 1:1 ion-association complexes, which resulted in a significant enhancement of RRS signal. The experimental conditions of HPLC and post-column RRS have been investigated, including detection wavelength, flow rate, pH, reacting tube length and reaction temperature. Reaction mechanism were studied in detail by calculating the distribution fraction. The maximum RRS signals for GFLX and SPLX were recorded at λex = λem = 330 nm. The detection limits were 3.8 ng ml−1 for GFLX and 17.5 ng ml−1 for SPLX at a signal-to-noise ratio of 3. The developed method was successfully applied to the determination of GFLX and SPLX in water samples. Recoveries from spiked water samples were 97.56–98.85%.
Datum: 22.12.2017


Exploration of interaction of canthaxanthin with human serum albumin by spectroscopic and molecular simulation methods

The interaction between the food colorant canthaxanthin (CA) and human serum albumin (HSA) in aqueous solution was explored by using fluorescence spectroscopy, three-dimensional fluorescence spectra, synchronous fluorescence spectra, UV–vis absorbance spectroscopy, circular dichroism (CD) spectra and molecular docking methods. The thermodynamic parameters calculated from fluorescence spectra data showed that CA could result in the HSA fluorescence quenching. From the KSV change with the temperature dependence, it was concluded that HSA fluorescence quenching triggered by CA is the static quenching and the number of binding sites is one. Furthermore, the secondary structure of HSA was changed with the addition of CA based on the results of synchronous fluorescence, three-dimensional fluorescence and CD spectra. Hydrogen bonds and van der Waals forces played key roles in the binding process of CA with HSA, which can be obtained from negative standard enthalpy (ΔH) and negative standard entropy (ΔS). Furthermore, the conclusions were certified by molecular docking studies and the binding mode was further analyzed with Discovery Studio. These conclusions can highlight the potential of the interaction mechanism of food additives and HSA.
Datum: 18.12.2017


Sodium 4-mercaptophenolate capped CdSe/ZnS quantum dots as a fluorescent probe for pH detection in acidic aqueous media

Development of the fluorescent pH detection method is promising due to the sensitivity, easy operation, and low-cost, etc. However, traditional organic fluorophores have still some disadvantages such as the tedious preparation and purification as well as low photostability and water solubility, which limits the rapid detection application. Semiconductor quantum dots (QDs) have recently risen to prominence as an alternative for organic fluorophores in fluorescence analysis by virtue of their convenient synthesis and superior optical properties. In this study, we report on sodium 4-mercaptophenolate functionalized CdSe/ZnS QDs (denoted as −OPhS-QDs), which can serve as a selective “on–off” fluorescence probe for aqueous media pH. −OPhS-QDs exhibit strong fluorescence in near neutral medium. As a Lewis organic base, −OPhS- moieties on QDs surface easily binds to proton under acidic conditions to yield 4-mercaptophenol capped QDs (i.e. HOPhS-QDs), which acts as an efficient hole trapper. As a result, the QDs photoluminescence (PL) is switched off. Under optimal conditions, the present probe exhibits a good linear relationship between fluorescence response and pH values in the pH range 3.0–5.2. Furthermore, the present probe exhibits a high selectivity for proton over other common cations and has been successfully used for pH detection in real water samples.
Datum: 13.12.2017


Study of the controlled assembly of DNA gated PEI/Chitosan/SiO2 fluorescent sensor

In this paper, polyethylenimine (PEI) and Chitosan were simultaneously one-step doped into silicon dioxide (SiO2) nanoparticles to synthesize PEI/Chitosan/SiO2 composite nanoparticles. The polymer PEI contained a large amount of amino groups, which can realize the amino functionalized SiO2 nanoparticles. And, the good pore forming effect of Chitosan was introduced into SiO2 nanoparticles, and the resulting composite nanoparticles also had a porous structure. In pH 7.4 phosphate buffer solution (PBS), the amino groups of PEI had positive charges, and therefore the fluorescein sodium dye molecule can be loaded into the channels of PEI/Chitosan/SiO2 composite nanoparticles by electrostatic adsorption. Furthermore, utilizing the diversity of DNA molecular conformation, we designed a high sensitive controllable assembly of DNA gated fluorescent sensor based on PEI/Chitosan/SiO2 composite nanoparticles as loading materials. The factors affecting the sensing performance of the sensor were investigated, and the sensing mechanism was also further studied.
Datum: 13.12.2017


Luminescent studies of binuclear ternary europium(III) pyridineoxide tetrazolate complexes containing bis-phosphine oxide as auxiliary co-ligands

A new class of antenna chromophores so called ‘tetrazolates’ have not been explored much for lanthanide luminescencent complexes. However, we have already published several articles considering pyridineoxide tetrazolates as sensitizer with lanthanide ions. Although this class of antenna attracted much less attention because of its poor photoluminescence quantum yields (tris-pyridineoxide tetrazolate europium complex = 13% in solution) we tried and successfully achieved to improve the photoluminescence quantum yields for this particular antenna molecule by replacing coordinated water from the inner coordination sphere of europium ion by introducing phosphine oxides as additional chromophore. In the present article the two bis-phosphine oxides attach two molecules of tris-pyridineoxide tetrazolate europium(III) complex which leads to the improvement of the overall molar absorption coefficients as well as photo-physical properties of the complexes. We found more than two-fold increase (31% in solution) in photoluminescence quantum yield with one of the coordinated phosphine oxides comparing with that of tris-pyridineoxide tetrazolate europium(III) complex.
Datum: 27.11.2017


Photoluminescent properties of Eu3+-Eu2+ activated MAl2SixO2x + 4 (M = Mg, Ca, Sr, Ba) phosphors prepared in air

In this paper, MAl2SixO2x+4:Eu2+/Eu3+ (Eu2+ + Eu3+ = 2%, molar ratio; M = Mg, Ca, Sr, Ba; x = 0, 0.5, 1, 1.5, 2) phosphors with different SiO2 concentrations (the ratio of SiO2 to MAl2O4 is n%, n = 0, 50, 100, 150, 200, respectively) were prepared by high-temperature solid-state reaction under atmospheric air conditions. Their structures and photoluminescent properties were systematically researched. The results indicate that Eu3+ ions have been reduced and Eu2+ ions are obtained in air through the self-reduction mechanism. The alkaline earth metal ions and doping SiO2 strongly affect the crystalline phase and photoluminescent properties of samples, including microstructures, relative intensity of Eu2+ to Eu3+, location of emission lines/bands. It is interesting and important that the emission color and intensities of europium-doped various phosphors which consist of aluminosilicate matrices prepared under atmospheric air conditions could be modulated by changing the kinds of alkaline earth metal and the content of SiO2.
Datum: 27.11.2017


Simeprevir oxidative degradation product: Molecular modeling, in silico toxicity and resolution by synchronous spectrofluorimetry

In this article, one of the potential degradation products of the novel antiviral drug simeprevir was isolated and characterized by means of infrared (IR) and mass spectrometry. Moreover, comparative molecular docking, ADMET (absorption, distribution, metabolism, excretion – toxicity) and insilico toxicity prediction studies were applied to evaluate the activity of simeprevir and its degradation product. Furthermore,a simple, accurate and selective second derivative synchronous spectrofluorimetric method was developed for the determination of simeprevir in the presence of its oxidative degradation product.The synchronous fluorescence spectra of both compounds were measured in ethanol at pH 2.0 usingΔλ of 140 nm and the peak amplitude of the second derivative spectra were measured at 442 nm. The method was rectilinear over the concentration range of 0.2 to 2.0 μg/ml and validated according to the ICH (International Conference on Harmonization) guidelines. Moreover, the method was statistically compared to the reverse-phase high-performance liquid chromatography (RP-HPLC) method and good results were obtained.
Datum: 10.11.2017


Utility of Cremophor RH 40 as a micellar improvement for spectrofluorimetric estimation of sorafenib in pure form, commercial preparation, and human plasma

An easy, quick, simple and accurate spectrofluorimetric method was recognized and validated for evaluation of sorafenib (SOR) in pure form and biologically in plasma. Cremophor RH 40 (Cr RH 40) used for enhancing the fluorescence activity of SOR in phosphate buffer (pH 7). Cr RH 40 improved the native fluorescence of SOR remarkably in water. The fluorescence spectrum of SOR was observed at 405 nm after excitation at 265 nm. The linearity appeared to be in the range of 5 to 600 ng ml−1 for pure and from 9 to 500 ng ml−1 for plasma using the protein precipitation (ppt) method while from 10 to 500 ng ml−1 for plasma using liquid–liquid extraction method. The precisions and the accuracy of the estimated method gave satisfactory results. The recommended method was effectively applied for determination of SOR in human plasma with high recovery values. The results of some compounds that are possibly found in plasma were studied. The proposed method was also focused on real volunteers and a drug dissolution test.
Datum: 10.11.2017


Enhancement effect on the chemiluminescence of acridinium esters under neutral conditions

Enhancement effect on the chemiluminescence of acridinium ester derivatives under neutral conditions was investigated. Additions of phenols did not enhance the chemiluminescence intensities of acridinium ester derivatives in the presence of horseradish peroxidase and hydrogen peroxide. Additions of cetyltrimethylammonium bromide apparently enhanced the chemiluminescence intensities of phenyl 10-methyl-10λ4-acridine-9-carboxylate derivatives with electron-withdrawing groups at the 4-position of the phenyl group. In particular, the chemiluminescence intensity of 4-(trifluoromethyl)phenyl 10-methyl-10λ4-acridine-9-carboxylate trifluoromethanesulfonate salt was 5.5 times stronger in the presence of cetyltrimethylammonium bromide than in its absence at pH 7. The chemiluminescence intensity of 3,4-dicyano-phenyl 10-methyl-10λ4-acridine-9-carboxylate trifluoromethanesulfonate salt was 46 times stronger in the presence of cetyltrimethylammonium bromide at pH 7 than in its absence at pH 10.
Datum: 08.11.2017


Spectroscopic determination of succinylcholine in dosage forms using eosin Y

Two simple and sensitive analytical assay methods using spectrophotometry and spectrofluorimetry techniques were developed for the estimation of succinylcholine chloride (SUC) in pharmaceutical preparations. The suggested methods are based on the formation of an ion pair complex formed between the drug and eosin Y spectrophotometrically (Method I), or the suppressive effect of succinylcholine on the native fluorescence property of eosin Y (Method II). The spectrophotometric method (Method I) involves measuring the absorbance of the complex between succinylcholine and eosin Y at 550 nm in Britton Robinson buffer of pH 3. However, the spectrofluorimetric method (Method II) involves measuring the quenching effect of the studied drug on the native fluorescence property of eosin Y at the same pH at 550 nm after excitation at 480 nm. The absorbance versus concentration of the drug is rectilinear over the range of 0.5 to 15 μg/ml. The formation constant was 3.5 × 104 and the Gibb's free energy change was −2.5 × 104 J/mol. In Method II, the relative fluorescence intensity was directly proportional to SUC concentration over the range of 0.05 to 1 μg/ml. The proposed methods allowed a successful application to the estimation of succinylcholine ampoules. An explanation of the reaction pathway was postulated.
Datum: 08.11.2017


A sensitive fluorescence quenching method for the detection of tartrazine with acriflavine in soft drinks

In this work, a simple, rapid, sensitive, selective spectrofluorimetric method was applied to detect tartrazine. The fluorescence of acriflavine could be efficiently quenched by tartrazine. The method manifested real time response as well as presented satisfied linear relationship to tartrazine. The linear response range of tartrazine (R2 = 0.9995) was from 0.056 to 5 μmol L−1. The detection limit (3σ/k) was 0.017 μmol L−1, indicating that this method could be applied to detect traces of tartrazine. The accuracy and precision of the method was further assured by recovery studies via a standard addition method, with percentage recoveries in the range of 96.0% to 103.0%. Moreover, a quenching mechanism was investigated systematically by the linear plots at varying temperatures based on the Stern–Volmer equation, fluorescence lifetime and UV–visible absorption spectra, all of which proved to be static quenching. This sensitive, selective assay possessed a great application prospect for the food industry owing to its simplicity and rapidity for the detection of tartrazine.
Datum: 02.11.2017


Fabrication of nitrogen- and phosphorous-doped carbon dots by the pyrolysis method for iodide and iron(III) sensing

A facile and novel strategy to synthesize nitrogen- and phosphorous-doped carbon dots (NPCDs) by single step pyrolysis method is described here. Citric acid is used as carbon source and di-ammonium hydrogen phosphate is used as both nitrogen and phosphorous sources, respectively. Through the extensive study on optical properties, morphology and chemical structures of the synthesized NPCDs, it is found that as-synthesized NPCDs exhibited good excitation-dependent luminescence property, spherical morphology and high stability. The obtained NPCDs are stable in aqueous medium and possess a quantum yield of 10.58%. In this work, a new assay method is developed to detect iodide ions using the synthesized NPCDs. Here, the inner filter effect is applied to detect the iodide ion and exhibited a wide linear response concentration range (10–60 μM) with a limit of detection (LOD) of 0.32 μM. Furthermore, the synthesized NPCDs are used for the selective detection of iron(III) (Fe3+) ions and cell imaging. Fe3+ ions sensing assay shows a detection range from 0.2 to 30 μM with a LOD of 72 nM. As an efficient photoluminescence sensor, the developed NPCDs have an excellent biocompatibility and low cytotoxicity, allowing Fe3+ ion detection in HeLa cells.
Datum: 02.11.2017


Comparison of the thermostability of recombinant luciferases from Brazilian bioluminescent beetles: Relationship with kinetics and bioluminescence colours

Firefly luciferases have been used extensively as bioanalytical reagents and their cDNAs as reporter genes for biosensors and bioimaging, but they are in general unstable at temperatures above 30°C. In the past few years, efforts have been made to stabilize some firefly luciferases for better application as analytical reagents. Novel luciferases from different beetle families, displaying distinct bioluminescence colours and kinetics, may offer desirable alternatives to extend the range of applications. In the past years, our group has cloned the largest variety of luciferases from the three main families of bioluminescent beetles (Elateridae: P. termitilluminans, F. bruchi, P. angustus; Phengodidae: P. hirtus, P. vivianii; and Lampyridae: A. vivianii, C. distinctus and Macrolampis sp2) occurring in Brazilian biomes. We compared the thermostability of these recombinant luciferases and investigated their relationships with bioluminescence spectra and kinetics. The most thermostable luciferases were those of Pyrearinus termitilluminans larval click beetle (534 nm), Amydetes vivianii firefly (539 nm) and Phrixotrix vivianii railroad worm (546 nm), which are the most blue-shifted examples in each family, confirming the trend that the most blue-shifted emitting luciferases are also the most thermostable. Comparatively, commercial P. pyralis firefly luciferase was less thermostable than P. termitilluminans click beetle and A. vivianii firefly luciferases. The higher thermostability in these luciferases could be related to higher degree of hydrophobic packing and disulfide bond content (for firefly luciferases).
Datum: 02.11.2017


A novel spectrofluorimetric method for determination of imatinib in pure, pharmaceutical preparation, human plasma, and human urine

The following paper represents a simple, highly sensitive, responsive validated and developed spectrofluorimetric method for estimation of imatinib (IMB) in its pure, commercial preparation, human urine and human blood plasma. The calibration curve was in the range 4–900 ng ml−1 for pure form and urine and 8–900 ng ml−1 for plasma in a medium contains carboxymethyl cellulose (CMC) and acetate buffer (pH 5) with excitation wavelength (λex) 230 nm and emission wavelength (λem) 307 nm. The limit of detection (LOD) was 0.37 ng ml−1 for the pure form, 0.64 ng ml−1 for human urine, and 0.70 ng ml−1 for human plasma, while the limit of quantitation (LOQ) was 1.2 for pure form, 1.91 for urine and 2.1 for plasma. The suggested method was successfully applied for evaluation of IMB in tablets within 99% mean percentage recovery. The excipients that are usually used as additives in pharmaceutical dosage form did not interfere with the suggested method. The method was efficiently used for estimation of IMB in human urine and human plasma. The effect of some cations that might be present in urine and plasma was also studied. The method was also focused on human volunteers and in vitro drug release.
Datum: 02.11.2017


Simultaneous determination of amlodipine and metoprolol in their combined dosage form using a synchronous fluorescence spectrofluorimetric method

Highly sensitive, rapid, accurate and precise synchronous fluorescence spectrofluorimetric method has been developed for simultaneous analysis of a mixture of amlodipine (AMD) and metoprolol (MET). The method relies on measuring the relative synchronous fluorescence intensity of both drugs at Δλ of 90 nm in acetate buffer solution at pH 5. The experimental parameters influencing the developed method were investigated and optimized. The method was linear over the ranges 0.2–2 μg/ml and 0.5–10 μg/ml for AMD and MET, respectively. The limits of detection were 50 ng/ml for AMD and 130 ng/ml for MET while the limits of quantitation were 150 ng/ml for AMD and 390 ng/ml for MET. The developed method was applied successfully for the determination of the two drugs in their co-formulated tablet. The mean percent recoveries were found to be 100.51 and 99.57 for AMD and MET, respectively.
Datum: 26.10.2017


Synthesis and characterization of KCe(PO3)4 doped with some lanthanide activators

KCe(PO3)4 doped with Dy3+,Tb3+,Yb3+and Nd3+ phosphors were synthesized by a solid state diffusion method. The prepared samples were characterized by X-ray diffraction and photoluminescence. KCe(PO3)4 exhibits emission in ultraviolet (UV) region which indicates weak Ce3+–Ce3+ interaction. The Ce3+–Ce3+energy transfer is not efficient. In light of this, energy transfer from Ce3+ to other lanthanides like Dy3+, Tb3+,Yb3+ and Nd3+ is rather surprising.
Datum: 26.10.2017


A carbonothioate-based highly selective fluorescent probe with a large Stokes shift for detection of Hg2+

Mercury (Hg) is one of the heavy metal pollutants in the environment. Even a very small amount of mercury can cause serious harm to human beings. Herein, we reported a new carbonothioate-based fluorescent probe for the detection of Hg2+ without interference from other metal ions. This probe possessed a very large Stokes shift (192 nm), which could improve the detection sensitivity by minimizing the interferences resulted from self-absorption or auto-fluorescence. With the addition of Hg2+ to the probe solution, considerable fluorescence enhancement was observed. Additionally, the Hg2+ concentration of 0–16 μM and fluorescence intensity showed a good linear relationship (y = 22106× + 53108, R2 = 0.9955). Finally, the proposed probe was used to detect Hg2+ in real water samples, and its result was satisfactory. Therefore, our proposed probe would provide a promising method for the determination of Hg2+ in the environment.
Datum: 25.10.2017


The determination of nitrite by a graphene quantum dot fluorescence quenching method without sample pretreatment

A method for quantitative analysis of nitrite was achieved based on fluorescence quenching of graphene quantum dots. To obtain reliable results, the effects of pH, temperature and reaction time on this fluorescence quenching system were studied. Under optimized conditions, decrease in fluorescence intensity of graphene quantum dots (F0/F) showed a good linear relationship with nitrite concentration between 0.007692–0.38406 mmol/L and 0.03623–0.13043 μmol/L; the limits of detection were 9.8 μmol/L and 5.4 nmol/L, respectively. Variable temperature experiments, UV absorption spectra and thermodynamic calculations were used to determine the quenching mechanism, and indicated that it was an exothermic, spontaneous dynamic quenching process. This method was used to analyse urine samples, and showed that it could be applied to analyse biological samples.
Datum: 25.10.2017


Enhanced red emission from BaMoO4: Eu3+ by Bi3+ co-doping

A series of Bi3+,Eu3+-doped BaMoO4 phosphors was synthesized using a hydrothermal method. The crystal structure, morphology and optical properties of the phosphors were studied using X-ray diffraction (XRD), scanning electron microscope (SEM) and photoluminescence (PL) measurements. Three different particle morphologies were detected in the SEM observation. The energy dispersive spectroscopy (EDS) results indicated that the solubility of Bi3+ in spherical or rugby-like BaMoO4 particles was very low and the excess Bi3+ element was cumulated in the irregular particles. Characteristic emissions of Eu3+ ions (5D0  7FJ; J = 0, 1, 2, 3, 4) were observed under excitation in ultraviolet (UV) light, with the most intense transition being the 5D0  7F2 transition. Energy transfer from MoO42− and Bi3+ to Eu3+ can be readily achieved. Red emission intensity of Eu3+ was enhanced by a factor of two by co-doping with a small amount of Bi3+. Optical properties as a function of Bi3+ content were studied and the optimum Bi3+ content in BaMoO4 nanocrystals was determined to be 0.4 mol%.
Datum: 23.10.2017


A comparative study on intrinsic fluorescence of BSA and lysozyme proteins in presence of different divalent ions from their solution and thin film conformations

Optical emission behaviours of lysozyme and bovine serum albumin, from bulk and thin film geometry, were studied in the presence of three different divalent ions (Mg2+, Ca2+ or Ba2+) using different spectroscopic [steady-state fluorescence, UV–Vis and Fourier transform infra-red (FTIR)] techniques. Additionally, protein thin films on silicon surfaces were prepared and morphological studies were carried out using atomic force microscopy. Dynamic quenching was mainly identified for both proteins in the presence of Mg2+, Ca2+ and Ba2+ ions. The molecular conformation of the proteins was modified in thin films compared with that in solution, consequently quenching efficiencies also varied. ATR-FTIR studies confirmed the conformational changes of proteins in the presence of all divalent ions. All metal ions used were divalent in nature and belonged to the same group of the periodic table but, depending on their individual characteristics such as electron affinity, ionic radius, etc., the magnitude of the protein and hydrated ion interaction varied and accordingly the quenching efficiency was modified. Quenching was maximum for Ca2+ ions, followed by the other two ions. Our study clearly illustrates the geometry-dependent physical and biological functions of proteins.
Datum: 20.10.2017


Spectroscopic investigation of the anticancer alkaloid piperlongumine binding to human serum albumin from the viewpoint of drug delivery

Piperlongumine (PL) is a very promising natural agent with a high potential for cancer treatment. To overcome the poor water solubility of PL, there is a need to develop a novel water-soluble formulation in which PL is non-covalently bound to human serum albumin (HSA). PL binding to HSA was studied by various spectroscopic techniques under simulated physiological conditions. Spectroscopic evidence showed that the interaction of PL with HSA could form a PL–HSA complex. The binding constant (Ka) values increased with increasing temperature, and a similar dependence was observed for the number of binding sites (n) values. The number of PL molecules bound to HSA reached 8.1 when the temperature was raised to 308 K. Thermodynamic calculation results suggested that the binding reaction occurred spontaneously but was an entropy-driven process, and hydrophobic forces played a major role in stabilizing the complex. Furthermore, PL binding induced conformational and microenvironmental changes in HSA. Displacement studies indicated that PL and warfarin had separate binding regions in site I. Therefore, it would be possible to develop a novel water-soluble formulation involving PL and HSA. This study may provide some valuable information in terms of improving the poor water solubility of PL.
Datum: 18.10.2017


Highly luminescent nitrogen-doped carbon dots for simultaneous determination of chlortetracycline and sulfasalazine

Here, we have presented a green and facile strategy to fabricate nitrogen-doped carbon dots (N-CDs) and their applications for determination of chlortetracycline (CTC) and sulfasalazine (SSZ). The fluorescent N-CDs, prepared by one-step hydrothermal reaction of citric acid and l-arginine, manifested numerous excellent features containing strong blue fluorescence, good water-solubility, narrow size distribution, and a high fluorescence quantum yield (QY) of 38.8%. Based on the fluorescence quenching effects, the as-synthesized N-CDs as a fluorescent nanosensor exhibited superior analytical performances for quantifying CTC and SSZ. The linear range for CTC was calculated to be from 0.85 to 20.38 μg ml−1 with a low detection limit of 0.078 μg ml−1. Meanwhile, the linear range for SSZ was estimated to be from 0.34 to 6.76 μg ml−1 with a low detection limit of 0.032 μg ml−1. Therefore, the N-CDs hold admirable application potential for constructing a fluorescent sensor for pharmaceutical analysis.
Datum: 18.10.2017


Synthesis and characterization of high quantum yield and oscillator strength 6-chloro-2-(4-cynophenyl)-4-phenyl quinoline (cl-CN-DPQ) organic phosphor for solid-state lighting

A novel blue luminescent 6-chloro-2-(4-cynophenyl) substituted diphenyl quinoline (Cl-CN DPQ) organic phosphor has been synthesized by the acid-catalyzed Friedlander reaction and then characterized to confirm structural, optical and thermal properties. Structural properties of Cl-CN-DPQ were analyzed by Fourier transform infrared (FTIR), nuclear magnetic resonance (NMR) spectroscopy, X-ray diffraction technique (XRD) and scanning electron microscopy (SEM) and energy dispersive analysis of X-ray (EDAX) spectroscopy. FTIR spectra confirmed the presence of different functional groups and bond stretching. 1H–NMR and 13C–NMR confirmed the formation of an organic Cl-CN-DPQ compound. X-ray diffraction study provided its crystalline nature. The surface morphology of Cl-CN-DPQ was analyzed by SEM, while EDAX spectroscopy revealed the elemental analysis. Differential thermal analysis (TGA/DTA) disclosed its thermal stability up to 250°C. The optical properties of Cl-CN-DPQ were investigated by UV–vis absorption and photoluminescence (PL) measurements. Cl-CN-DPQ exhibits intense blue emission at 434 nm in a solid-state crystalline powder with CIE co-ordinates (0.157, 0.027), when excited at 373 nm. Cl-CN-DPQ shows remarkable Stokes shift in the range 14800–5100 cm−1, which is the characteristic feature of intense light emission. A narrow full width at half-maximum (FWHM) value of PL spectra in the range 42–48 nm was observed. Oscillator strength, energy band gap, quantum yield, and fluorescence energy yield were also examined using UV–vis absorption and photoluminescence spectra. These results prove its applications towards developing organic luminescence devices and displays, organic phosphor-based solar cells and displays, organic lasers, chemical sensors and many more.
Datum: 18.10.2017


Nitrogen-doped carbon dots as a fluorescent probe for the highly sensitive detection of Ag+ and cell imaging

An easy hydrothermal synthesis strategy was applied to synthesize green-yellow emitting nitrogen-doped carbon dots (N-CDs) using 1,2-diaminobenzene as the carbon source, and dicyandiamide as the dopant. The nitrogen-doped CDs resulted in improvement in the electronic characteristics and surface chemical activities. N-CDs exhibited bright fluorescence emission and could response to Ag+ selectively and sensitively. Other ions produced nearly no interference. A N-CDs based fluorescent probe was then applied to sensitively determine Ag+ with a detection limit of 5 × 10−8 mol/L. The method was applied to the determination of Ag+ dissolved in water. Finally, negligibly cytotoxic, excellently biocompatibile, and highly fluorescent carbon dots were applied for HepG2 cell imaging and the quenched fluorescence by adding Ag+, which indicated its potential applications.
Datum: 18.10.2017


Colorimetric detection of oxalate in aqueous solution by a pyrogallol red-based Cu2+ complex

The pyrogallol red (PR)-based Cu2+ complex was proven to be an effective and selective colorimetric chemosensing ensemble for recognition of oxalate over other anions in a perfect aqueous solution. The addition of oxalate to the PR–Cu2+ complex resulted in a colour change from purple to orange colour due to the regeneration of PR by the chelation of oxalate with Cu2+, while other anions did not induce any significant colour change. Moreover, it was revealed that no obvious interference was observed during the titrations with oxalate into each other anion. Therefore, the PR–Cu2+ complex can be used as a simple and practical colorimetric chemosensor for detecting oxalate.
Datum: 18.10.2017


Synthesis of carbon-based quantum dots from starch extracts: Optical investigations

Carbon-based quantum dots (C-QDs) were synthesized through microwave-assisted carbonization of an aqueous starch suspension mediated by sulphuric and phosphoric acids. The as-prepared C-QDs showed blue, green and yellow luminescence without the addition of any surface-passivating agent. The C-QDs were further analyzed by UV−vis spectroscopy to measure the optical response of the organic compound. The energy gaps revealed narrow sizing of C-QDs in the semiconductor range. The optical refractive index and dielectric constant were investigated. The C-QDs size distribution was characterized. The results suggested an easy route to the large scale production of C-QDs materials.
Datum: 12.10.2017


Probing the interaction of cephalosporin with bovine serum albumin: A structural and comparative perspective

Cephalosporins belong the largest class of antibiotics used in the treatment of a wide range of infectious diseases caused by susceptible organisms. In the present study, we chose two typical antibiotics cefalexin/cefixime based on their structure, and investigated the interaction of cephalexin/cefixime with bovine serum albumin (BSA) using UV–vis absorption spectra, fluorescence spectroscopy, circular dichroism (CD) spectroscopy and molecular modeling approaches. Spectroscopic experiments revealed the formation of a BSA − cefalexin/cefixime complex. The binding parameters calculated using a modified Stern − Volmer method and the Scatchard method reached 103–104 L·mol−1. Thermodynamic parameter studies revealed that binding characteristics by negative enthalpy and positive entropy changes, and electrostatic interactions play a major role. Site marker competitive displacement experiments and molecular modeling approaches demonstrated that cefalexin and cefixime bind with appropriate affinity to site I (subdomain IIA) of BSA. Furthermore, synchronous fluorescence spectra, CD spectra and molecular modeling results indicated that the secondary structure of BSA was changed in the presence of cefalexin and cefixime. Additionally, the effects of metal ions on the BSA − cefalexin/cefixime system were also assessed.
Datum: 04.10.2017


Application of quinone-based fluorophore and native fluorescence for the spectrofluorimetric determination of agomelatine in dosage form: Identification of acidic and alkaline- induced degradation products by LC–MS/TOF

Three spectrofluorimetric methods were developed for agomelatine (AGM) determination in commercial tablets. Method A is based on measuring the native fluorescence of AGM aqueous solution at 230/360 nm. Methods B and C are based on the formation of a charge transfer complex between AGM and 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ) and 7,7,8,8-tetracyanoquinodimethane (TCNQ) with measurement of the formed fluorophore at 365/475 nm and 250/304 nm, respectively. The relative fluorescence intensity (RFI) of AGM–DDQ complex was greatly enhanced in the presence of methyl-β-cyclodextrin (CD). The methods were linear over the concentration ranges of 0.015–0.5, 0.5–8.0, 0.09–6.0 and 0.05–0.2 μg/ml for AGM-native fluorescence, AGM–DDQ, AGM–DDQ–CD and AGM–TCNQ complexes, respectively with excellent correlation coefficients (r = 0.9999). The methods were validated as per the International Conference on Harmonization (ICH) guidelines and all validation requirements were satisfied. The developed methods were extended to the analysis of AGM in commercial tablets. Furthermore, the stability of AGM was studied under different stress conditions (alkaline, acidic, oxidative and photolytic). The potential alkaline and acidic degradation products were identified by LC–MS/TOF.
Datum: 02.10.2017


Selective fluorescence quenching of papain–Au nanoclusters by self-polymerization of dopamine

In this paper, we synthesized a papain-stabilized fluorescent Au nanocluster (NC) probe and studied its interaction with dopamine. As fluorescence of papain–Au NCs is quenched in the presence of dopamine under alkaline conditions, we were able to establish a simple, selective analytical method for dopamine determination. By studying the fluorescence lifetime and dynamic light scattering of the NCs before and after interaction with dopamine, we found that this fluorescence quenching mechanism was possibly due to dopamine self-polymerization that produced polydopamine that cross-linked papain–Au NCs. Based on this new phenomenon, we proposed a highly selective analytical method for dopamine detection. Other small organic molecules, such as amino acids, ascorbic acid and uric acid did not interfere with dopamine detection. Dopamine in the range 20–100 μM can be linearly detected by the fluorescence quenching ratio of gold nanoclusters. Dopamine detection could be visually realized by watching color changes of papain–Au NCs under UV light or daylight, as both fluorescence and absorption of the papain–Au NCs changed during dopamine polymerization.
Datum: 28.09.2017


Reviving near infra-red emission of Ag2S nanoparticles using interfacial defects in the Ag2S@CdS core–shell structure

Ag2S@CdS core–shell particles were synthesized with different Cd source content as a measure of shell thickness using a pulsed microwave irradiation method. The particles were verified structurally using X-ray diffraction, energy dispersive X-ray analysis and transmission electron microscopy. Optical spectroscopy revealed that core–shells show an absorption peak at 750 nm and an emission peak located around 800 nm after 6 min of microwave irradiation. With continued microwave treatment, the NIR luminescence first vanished but it was revived after 12 min of irradiation, which was 100 nm red shifted. This new type of NIR emission in Ag2S with sizes greater than 5 nm is due to the proximity of a highly deficient CdS shell with strong red emission that was stable for more than 6 months in water. A mechanism has been suggested for this type of emission.
Datum: 28.09.2017


Development of an optical sensor for chlortetracycline detection based on the fluorescence quenching of l-tryptophan

A simple and selective spectrofluorimetric method for the detection of chlortetracycline (CTC) was studied. In pH 7.4 buffer medium l-tryptophan (l-Trp), applied as the fluorescence probe, interacted with CTC resulting in fluorescence quenching of the probe. CTC was detected with maximum excitation and emission wavelengths at λex/λem = 275/350 nm. Notably, quenching of fluorescence intensities was positively proportional to the CTC concentration over the range of 0.65–30 μmol L−1 and the limit of detection was 0.2 μmol L−1. Effect of temperature shown in Stern−Volmer plots, absorption spectra and fluorescence lifetime determination, indicated that fluorescence quenching of l-Trp by CTC was mainly by static quenching. The proposed study used practical samples analysis satisfactorily.
Datum: 20.09.2017


A selective and sensitive fluorescent sensor for cysteine detection based on bi-8-carboxamidoquinoline derivative and Cu2+ complex

In this paper, a novel fluorescent sensor 1 for selective and sensitive detection of cysteine was developed based on a complex between bi-8-carboxamidoquinoline derivative ligand (L) and Cu2+. The interaction of Cu2+ with the ligand causes a dramatic fluorescence quenching most likely due to its high affinity towards Cu2+ and a ligand–metal charge transfer (LMCT) process. The in situ generated L–Cu2 complex was utilized as a chemosensing ensemble for cysteine. In the presence of cysteine, the fluorophore, L, was released from L–Cu2 complex because of the strong affinity of cysteine to Cu2+ via the Cu–S bond, leading to the fluorescence recovery of the ligand. The proposed displacement mechanism was confirmed by the results of mass spectrometry (MS) study. Under optimized conditions, the recovered fluorescence intensity is linear with cysteine concentrations in the range 1 × 10−6 mol/l to 8 × 10−6 mol/l. The detection limit for cysteine is 1.92 × 10−7 mol/l. Furthermore, the established method showed a highly sensitive and selective response to cysteine among the 20 fundamental α-amino acids used as the building blocks of proteins, after Ni2+ was used as a masking agent to eliminate the interference of His. The proposed sensor is applicable in monitoring cysteine in practical samples with good recovery rate.
Datum: 20.09.2017


Evaluation of chemical chaperones based on the monitoring of Bip promoter activity and visualization of extracellular vesicles by real-time bioluminescence imaging

It is known that endoplasmic reticulum (ER) stress in cells and extracellular vesicles (EVs) plays a significant role in cancer cells, therefore the evaluation of compounds that can regulate ER stress and EV secretion would be a suitable system for further screening and development of new drugs. In this study, we evaluated chemical chaperones derived from natural products based on monitoring Bip/GRP78 promoter activity during cancer cell growth, at the level of the single cell, by a bioluminescence microscopy system that had several advantages compared with fluorescence imaging. It was found that several chemical chaperones, such as ferulic acid (FA), silybin, and rutin, affected the activity. We visualized EVs from cancer cells using bioluminescence imaging and showed that several EVs could be observed when using CD63 fused with NanoLuc luciferase, which has a much smaller molecular weight and higher intensity than conventional firefly luciferase. We then examined the effects of the chemical chaperones on EVs from cancer cells by bioluminescence imaging and quantified the expression of CD63 in these EVs. It was found that the chemical chaperones examined in this study affected CD63 levels in EVs. These results showed that imaging at the level of the single cell using bioluminescence is a powerful tool and could be used to evaluate chemical chaperones and EVs from cancer cells. This approach may produce new information in this field when taken together with conventional and classical methods.
Datum: 20.09.2017


In situ biosensor for detection miRNA in living cells based on carbon nitride nanosheets with catalytic hairpin assembly amplification

In this study, an ultrasensitive fluorescence turn-on assay for in situ sensing of intracellular microRNA (miRNA) was developed utilizing a carbon nitride nanosheet (CNNS) and a catalytic hairpin assembly (CHA). The CHA showed favourable signal amplification for low-level biomarkers, and CNNS was an excellent candidate as a fluorescence quencher and gene vector. Moreover, the hairpin DNA of CHA could be adsorbed onto the surface of CNNS. An enzyme-free fluorescence biosensor for ultrasensitive sensing of intracellular miRNA in cells based on CHA and CNNS was designed. When faced with target miRNA, the fluorescence was recovered due to the miRNA, which could trigger cycling of CHA circuits, leading to the production of a marked enhanced fluorescence signal. Compared with traditional methods, the proposed method is convenient, with low cytotoxicity, and high specificity and ultrasensitivity. It has promising potential for detection low-level biomarkers.
Datum: 20.09.2017


Boron and nitrogen co-doped carbon dots as a sensitive fluorescent probe for the detection of curcumin

In this present study, a fluorescent probe was developed to detect curcumin, which is derived from the rhizomes of the turmeric. We used a simple and economical way to synthesize boron and nitrogen co-doped carbon dots (BNCDs) by microwave heating. The maximum emission wavelength of the BNCDs was 450 nm at an excitation wavelength of 360 nm. The as-prepared BNCDs were characterized by multiple analytical techniques such as transmission electron microscopy, X-ray photoelectron spectroscopy, X-ray diffraction, and infrared spectroscopy. The synthesized carbon nanoparticles had an average particle diameter of 4.23 nm. The BNCDs exhibited high sensitivity to the detection of curcumin at ambient conditions. The changes of BNCDs fluorescent intensity show a good linear relationship with the curcumin concentrations in the range 0.2–12.5 μM. This proposed method has been successfully applied to detect the curcumin in urine samples with the recoveries of 96.5–105.5%.
Datum: 15.09.2017


Comparison of molecular interactions of Ag2Te and CdTe quantum dots with human serum albumin by spectroscopic approaches

Ag2Te quantum dots (QDs) have attracted great attention in biological applications due to their superior photoluminescence qualities and good biocompatibility, but their potential biotoxicity at a molecular biology level has been rarely discussed. In order to better understand the basic behavior of Ag2Te QDs in biological systems and compare their biotoxicity to cadmium-containing QDs, a series of spectroscopic measurements was applied to reveal the molecular interactions of Ag2Te QDs and CdTe QDs with human serum albumin (HSA). Ag2Te QDs and CdTe QDs statically quenched the intrinsic fluorescence of HSA by electrostatic interactions, but Ag2Te QDs exhibited weaker quenching ability and weaker binding ability compared with CdTe QDs. Electrostatic interactions were the main binding forces and Sudlow's site I was the primary binding site during these binding interactions. Furthermore, micro-environmental and conformational variations of HSA were induced by their binding interactions with two QDs. Ag2Te QDs caused less secondary structural and conformational change in HSA, illustrating the lower potential biotoxicity risk of Ag2Te QDs. Our results systematically indicated the molecular binding mechanism of Ag2Te QDs with HSA, which provided important information for possible toxicity risk of these cadmium-free QDs to human health.
Datum: 14.09.2017


Redox luminescence switch based on Mn2+-doped NaYF4:Yb,Er upconversion nanorods

An redox luminescence switch was developed for the sensing of glutathione (GSH), l-cysteine (Cys) or l-ascorbic acid (AA) based on redox reaction. The Mn2+-doped NaYF4:Yb,Er upconversion nanorods (UCNRs) with an emission peak located in the red region were synthesized. The luminescence intensity of the UCNRs could be quenched due to the Mn2+ could be oxidized to MnO2 by KMnO4. Subsequently, when the AA, GSH or Cys was added into the MnO2 modified upconversion nanosystem, which could reduced MnO2 to Mn2+ and the luminescence intensity was recovered. The concentration ranges of the nanosystem are 0.500–3.375 mM (R2 = 0.99) for AA, 0.6250–11.88 mM (R2 = 0.99) for GSH and 0.6250–9.375 mM (R2 = 0.99) for Cys, respectively.
Datum: 07.09.2017


Rapid and sensitive colorimetric detection of ascorbic acid in food based on the intrinsic oxidase-like activity of MnO2 nanosheets

In this paper, we report a colorimetric sensor for the rapid, selective detection of ascorbic acid (AA) in aqueous solutions. Single-layered MnO2 nanosheets were established as an artificial oxidase; consequently colorless 3,3´,5,5´-tetramethylbenzidine (TMB) was oxidized to a blue product (oxTMB), with increase in absorbance at 650 nm. The absorbance of the reaction system decreased after introduction AA, which reduced MnO2 into Mn2+. Under optimum conditions, a detection limit of 62.81 nM for AA in aqueous solutions could be achieved. The linear response range for AA was 0.25–30 μM with a correlation coefficient of 0.996. Importantly, the MnO2 nanosheet–TMB chromogenic reaction exhibited great selectivity as there was no interference from other metal ions, amino acids and small biological molecules. The proposed colorimetric sensing of AA could be applied for fruit, juice and pharmaceutical samples. Moreover, the proposed sensor showed satisfying performance, including low cost, easy preparation, rapid detection, and good biocompatibility.
Datum: 30.08.2017


Terbium (III) coordination polymer–copper (II) compound as fluorescent probe for time-resolved fluorescence ‘turn-on’ detection of hydrogen sulfide

With recognition of the biological importance of hydrogen sulfide (H2S), we present a simple and effective fluorescent probe for H2S using a Tb3+ coordination polymer–Cu2+ compound (DPA/Tb/G–Cu2+). Dipicolinic acid (DPA) and guanosine (G) can coordinate with Tb3+ to form a macromolecular coordination polymer (DPA/Tb/G). DPA/Tb/G specifically binds to Cu2+ in the presence of coexisting cations, and obvious fluorescence quenching is observed. The quenched fluorescence can be exclusively recovered upon the addition of sulfide, which is measured in the mode of time-resolved fluorescence. The fluorescence intensities of the DPA/Tb/G–Cu2+ compound enhance linearly with increasing sulfide concentrations from 1 to 30 μM. The detection limit for sulfide in aqueous solution is estimated to be 0.3 μM (at 3σ). The DPA/Tb/G–Cu2+ compound was successfully applied to sense H2S in human serum samples and exhibited a satisfactory result. It displays some desirable properties, such as fast detection procedure, high selectivity and excellent sensitivity. This method is very promising to be utilized for practical detection of H2S in biological and environmental samples.
Datum: 30.08.2017


Silica-modified luminescent LaPO4:Eu@LaPO4@SiO2 core/shell nanorods: Synthesis, structural and luminescent properties

Monoclinic-type tetragonal LaPO4:Eu (core) and LaPO4:Eu@LaPO4 (core/shell) nanorods (NRs) were successfully prepared using a urea-based co-precipitation process under ambient conditions. An amorphous silica layer was coated around the luminescent core/shell NRs via the sol–gel process to improve their solubility and colloidal stability in aqueous and non-aqueous media. The prepared nano-products were systematically characterized by X-ray diffraction pattern, transmission electron microscopy, energy dispersive X-ray analysis, and FTIR, UV/Vis, and photoluminescence spectroscopy to examine their phase purity, crystal phase, surface chemistry, solubility and luminescence characteristics. The length and diameter of the nano-products were in the range 80–120 nm and 10–15 nm, respectively. High solubility of the silica-modified core/shell/Si NRs was found for the aqueous medium. The luminescent core NRs exhibited characteristic excitation and emission transitions in the visible region that were greatly affected by surface growth of insulating LaPO4 and silica layers due to the multiphonon relaxation rate. Our luminescence spectral results clearly show a distinct difference in intensities for core, core/shell, and core/shell/Si NRs. Highly luminescent NRs with good solubility could be useful candidates for a variety of photonic-based biomedical applications.
Datum: 17.08.2017


Preliminary results on the photoluminescence and optically stimulated luminescence in Cu-doped and Ag-doped ZnB2X4 (B = Li, Na, K: X = Cl, Br) compounds

Photoluminescence, and optically stimulated luminescence in ZnB2X4 (B; Li,Na,K: X; Cl,Br) compounds doped with Cu+ or Ag+ were studied. Double humped emission bands attributable to the activators were observed in all the samples. The observed photoluminescence of Cu+ and Ag+ could be identified with 3d94s13d10 and 4d95s15d10 transitions respectively. The longer wavelength band (400–500 nm range) could be attributed to the Cu+ or Ag+ ion replacing alkali ion at the octahedral alkali site whereas short wavelength band (340–400 nm range) is attributed to a Cu or Ag ion at tetrahedral zinc site. The short wavelength band was found to be intense compared with long wavelength and gave an indication that most of the Cu or Ag ions prefered a tetrahedral Zn site compared with the octahedral alkali site. All the samples exhibit optically stimulated luminescence (OSL). The sensitivity was found to be lattice dependent. The lowest sensitivity of about 1% compared with Al2O3:C was observed in lithium lattices whereas highest the sensitivity of about 290% was observed in the case of Cu-doped ZnNa2Br4.
Datum: 17.08.2017


Synthesis, density functional theory calculations and luminescence of lanthanide complexes with 2,6-bis[(3-methoxybenzylidene)hydrazinocarbonyl] pyridine Schiff base ligand

A pyridine-diacylhydrazone Schiff base ligand, L = 2,6-bis[(3-methoxy benzylidene)hydrazinocarbonyl]pyridine was prepared and characterized by single crystal X-ray diffraction. Lanthanide complexes, Ln–L, {[LnL(NO3)2]NO3.xH2O (Ln = La, Pr, Nd, Sm, Eu, Gd, Tb, Dy and Er)} were prepared and characterized by elemental analysis, molar conductance, thermal analysis (TGA/DTGA), mass spectrometry (MS), Fourier transform infra-red (FT-IR) and nuclear magnetic resonance (NMR) spectroscopy. Ln–L complexes are isostructural with four binding sites provided by two nitro groups along with four coordination sites for L. Density functional theory (DFT) calculations on L and its cationic [LnL(NO3)2]+ complexes were carried out at the B3LYP/6–31G(d) level of theory. The FT-IR vibrational wavenumbers were computed and compared with the experimentally values. The luminescence investigations of L and Ln–L indicated that Tb–L and Eu–L complexes showed the characteristic luminescence of Tb(III) and Eu(III) ions. Ln–L complexes show higher antioxidant activity than the parent L ligand.
Datum: 17.08.2017


A sensitive surface-enhanced Raman scattering method for chondroitin sulfate with Victoria blue 4R molecular probes in nanogold sol substrate

Using silver nanoparticles (AgNPs) as the nanocatalyst, l-cysteine rapidly reduced HAuCl4 to make a stable gold nanoparticle sol (Ag/AuNP) that had a high surface-enhanced Raman scattering (SERS) activity in the presence of Victoria blue 4R (VB4r) molecular probes. Under the selected conditions, chondroitin sulfate (Chs) reacted with the VB4r probes to form associated complexes that caused the SERS effect to decrease to 1618 cm−1. The decreased SERS intensity was linear to the Chs concentration in the range 3.1–500 ng/ml, with a detection limit of 1.0 ng/ml Chs. Accordingly, we established a simple and sensitive SERS quantitative analysis method to determine Chs in real samples, with a relative standard deviation of 1.47–3.16% and a recovery rate of 97.6–104.2%.
Datum: 11.08.2017


Effect of erbium ion concentration on structural and luminescence properties of lead borosilicate glasses for fiber amplifiers

This investigation reports, the effect of the concentration of erbium and lead ions on the physical, structural and optical properties of lead borosilicate glasses. These glasses were synthesized by the melt quench method. In the synthesis, the concentration of the erbium (Er3+) ion was varied in the order of 0.0, 0.1, 0.5, 1.0 and 2.0 mol% and lead (Pb2+) ion was varied in the order of 30, 29.9, 29.5, 29 and 28 mol%. The glasses were analyzed using X-ray diffraction (XRD), Fourier transform infrared (FT-IR) and UV–vis–NIR spectroscopy. From XRD, the amorphous nature of lead borosilicate glasses was confirmed. The functional groups which were present in the glasses have been identified by analyzing the FT-IR spectrum. From the absorption spectra, the oscillator strengths as well as the Judd–Ofelt (JO) intensity parameters were determined and compared with other hosts. The JO intensity parameters were further used to calculate certain radiative properties for the excited luminescent levels of Er3+ ion. From emission spectra, full width at half maxima (FWHM), stimulated emission cross-sections (σe) and certain lasing parameters were evaluated and compared with reference host glasses. The lifetimes of 4I13/2 excited level of Er3+ ion have also been recorded and analyzed. The calculated and experimental lifetimes were compared in terms of quantum efficiencies. From the photoluminescence analysis, the erbium doped lead borosilicate glasses well suited for optical fiber amplifiers are discussed.
Datum: 08.08.2017


Ratiometric fluorescence detection of superoxide anion based on AuNPs-BSA@Tb/GMP nanoscale coordination polymers

A novel ratiometric fluorescence nanosensor for superoxide anion (O2•−) detection was designed with gold nanoparticles-bovine serum albumin (AuNPs-BSA)@terbium/guanosine monophosphate disodium (Tb/GMP) nanoscale coordination polymers (NCPs) (AuNPs-BSA@Tb/GMP NCPs). The abundant hydroxyl and amino groups of AuNPs-BSA acted as binding points for the self-assembly of Tb3+ and GMP to form core-shell AuNPs-BSA@Tb/GMP NCP nanosensors. The obtained probe exhibited the characteristic fluorescence emission of both AuNPs-BSA and Tb/GMP NCPs. The AuNPs-BSA not only acted as a template to accelerate the growth of Tb/GMP NCPs, but also could be used as the reference fluorescence for the detection of O2•−. The resulting AuNPs-BSA@Tb/GMP NCP ratiometric fluorescence nanosensor for the detection of O2•− demonstrated high sensitivity and selectivity with a wide linear response range (14 nM–10 μM) and a low detection limit (4.7 nM).
Datum: 04.08.2017


Luminescent properties of single-phase Ba2-x-1.5y-1.5zP2O7:xEu2+,yCe3+, zTb3+ phosphors for white light-emitting diode

A series of Ba2P2O7:xEu2+,yCe3+,zTb3+ phosphors was synthesized via a co-precipitation method, then their crystal structure, quantum efficiency and luminescent properties were analyzed by XRD and FL, respectively. The results showed that these phosphors not only presented the excitation characteristics of Ba2P2O7:xEu2+,zTb3+, but also exhibited that of the Ba2P2O7:yCe3+,zTb3+ phosphor. Meanwhile, the tri-doped phosphor showed a stronger absorption around 320 nm in contrast with the Eu2+/Ce3+:Tb3+ co-doped phosphor. Not only can energy transfer from Ce3+Eu2+ be observed; the energy transfer mechanism from Eu2+ to Tb3+ is discussed in the tri-doped system. Ce3+ affects the luminescence properties of Ba2P2O7:xEu2+,yCe3+,zTb3+ phosphors just as the sensitizer whereas Eu2+ is considered both as the sensitizer and the activator. The chromaticity coordinates of tri-doped phosphors excited at 320 nm stayed steadily in the bluish-white light region,and the emitted color and color temperature (CCT) of these phosphors could be tuned by adjusting the relative contents of Eu2+, Ce3+ and Tb3+. Hence, the single phase Ba2P2O7:xEu2+,yCe3+,zTb3+ phosphors may be considered as potential candidates for white light-emitting diodes.
Datum: 01.08.2017


Improved sensitivity of firefly luminescent intermediate-based protein interaction assay using Ser 440 mutant with lower adenylation activity

Protein–protein interaction assays are important in various fields including molecular biology, diagnostics, and drug screening. We recently designed a novel protein–protein interaction assay, the firefly luminescent intermediate-based protein interaction assay (FlimPIA), that exploited the unique reaction mechanism of firefly luciferase (Fluc). Using two mutant Flucs, each impaired with one of the two half-reactions, namely adenylation and subsequent oxidative luminescent steps, FlimPIA detects the proximity of the two proteins tethered to the mutant Flucs. Here, we found that introducing a mutation into a residue in the hinge region (S440) of the mutant with lowered adenylation activity (‘Acceptor’ Fluc) further improved the response of FlimPIA by lowering the residual adenylation activity. Mutants with bulkier residues showed greater inhibition, probably due to increased steric hindrance at the adenylation conformation. As a result, the FlimPIA with S440 L acceptor showed the best signal/background ratio for the detection of rapamycin-induced FKBP12–FRB interactions.
Datum: 28.07.2017


Investigation of the interaction of aurantio-obtusin with human serum albumin by spectroscopic and molecular docking methods

The interaction between human serum albumin (HSA) and aurantio-obtusin was investigated by spectroscopic techniques combined with molecular docking. The Stern–Volmer quenching constants (KSV) decreased from 8.56 × 105 M−1 to 5.13 × 105 M−1 with a rise in temperatures from 289 to 310 K, indicating that aurantio-obtusin produced a static quenching of the intrinsic fluorescence of HSA. Time-resolved fluorescence studies proved again that the static quenching mechanism was involved in the interaction. The sign and magnitude of the enthalpy change as well as the entropy change suggested involvement of hydrogen bonding and hydrophobic interaction in aurantio-obtusin–HSA complex formation. Aurantio-obtusin binding to HSA produced significant alterations in secondary structures of HSA, as revealed from the time-resolved fluorescence, Fourier transform infrared (FT-IR) spectroscopy, three-dimensional (3D) fluorescence and circular dichroism (CD) spectral results. Molecular docking study and site marker competitive experiment confirmed aurantio-obtusin bound to HSA at site I (subdomain IIA).
Datum: 26.07.2017


Preparation of carbon dot-based ratiometric fluorescent probes for cellular imaging from Curcuma longa

This work derived biocompatible and stable probes based on fluorescent nanoparticles (FNPs) from a natural source, Curcuma longa. The multi-color fluorescence emissions from carbonized Curcuma longa (C-FNPs) obtained through defined dehydration conditions are soluble in water and have a small particle size (~17 nm). The surface passivation with polyethylene glycol (PEG) capped with amine groups in FNPs (P-FNPs) generated a probe with a higher quantum yield and longer fluorescence lifetime than obtained with C-FNPs. The X-ray photoelectron spectroscopy and X-ray diffraction spectra confirmed the associated chemical moieties of C-FNPs and P-FNPs. Furthermore, the prepared material showed non-toxic effects with almost 100% cell viability, even at high concentrations. In conclusion, fluorescence sensors from natural sources may be useful for numerous biomedical research applications.
Datum: 18.07.2017


Characterization of a highly Al3+-selective fluorescence probe based on naphthalimide-Schiff base and its application to practical water samples

A new fluorescent Al3+-probe, N-allyl-4-[3,3′-((2-aminoethyl)azanediyl)-bis(N´-(2-hydroxybenzylidene)propanehy-drazide)]-1,8-naphthalimide (L), was designed and synthesized based on 1,8-naphthalimide. The probe L contains 1,8-naphthalimide moiety as the fluorophore and a Schiff base as the recognition group. The structure of L was determined by single crystal X-ray. L emission at 526 nm increased on addition of Al3+ under excitation wavelength at 350 nm. L exhibited high selectivity and sensitivity fluorescence emission towards to Al3+ in ethanol/Tris–HCl buffer solution (1:1, v/v, pH = 7.2) as compared with other tested metal ions. A good linearity with a correlation coefficient (R2) of 0.99 was observed in the concentration range 2–10 μM. The binding constant and the detection limit of L for Al3+ were calculated to 2.6 × 104 M−1 and 0.34 μM, respectively. The results of experiments that including Job plot, ultraviolet–visible (UV–Vis) light titration, fluorescence titration, ESI-MS and 1H NMR titration, indicated a 1:1 stoichiometric complex between L and Al3+. L was highly effective in monitoring Al3+ in real-life Yellow River and tap water samples.
Datum: 18.07.2017


An electrochemiluminescent method for glutamate measurement in small microdialysate samples in asphyxiated young rats

Glutamate (Glu) quantification has been performed by a combination of intracerebral microdialysis through which the samples are obtained and analyzed by high performance liquid chromatography (HPLC); its measurement requires a large expenditure of time (15–30 min per sample) and special training. Therefore, an alternative method is presented here, based on the electrochemiluminescence produced by the use of an enzymatic reactor, containing glutamate-oxidase, mixed and incubated with microdialysate from dorsal striatum (DS) and prefrontal cortex (PFC) of young rats asphyxiated during the neonatal period, under a global asphyxia model in order to test this method. Using this approach, we found high extracellular Glu concentration in the DS of asphyxiated animals, but only during K+ stimulation, while in the PFC, only a delay in the rise of Glu after K+ stimulation was observed, without any difference in extracellular Glu content when compared with controls. This new method permitted a fast measurement of Glu in brain dialysate samples, it significantly reduces the cost of the analysis per sample, since only a single device and pump are needed without using columns and high pressure inside the system or complex hardware and software to control pumps, detector, fraction collector or any other peripheral used in HPLC.
Datum: 18.07.2017


A rhodamine-triazole fluorescent chemodosimeter for Cu2+ detection and its application in bioimaging

A rhodamine-based fluorescent chemodosimeter rhodamine hydrazide-triazole (RHT) tethered with a triazole moiety was developed for Cu2+ detection. In aqueous medium, the RHT probe exhibited high selectivity and sensitivity toward Cu2+ among other metal ions. The addition of Cu2+ triggered a fluorescence emission of RHT by 384-fold (Φ = 0.33) based on a ring-opening process and a subsequent hydrolysis reaction. Moreover, RHT also showed a selective colorimetric response toward Cu2+ from colorless solution to pink, readily observed with the naked eye. The limit of detection of RHT for Cu2+ was calculated to be 1 nM (0.06 ppb). RHT was successfully demonstrated to detect Cu2+ in Chang liver cells by confocal fluorescence microscopy.
Datum: 17.07.2017


Investigation on the pH-independent photoluminescence emission from carbon dots impregnated on polymer matrix

Highly luminescent, polymer nanocomposite films based on poly(vinyl alcohol) (PVA), and monodispersed carbon dots (C-dots) derived from multiwalled carbon nanotubes (MWCNTs), as coatings on substrates as well as free standing ones are obtained via solution-based techniques. The synthesized films exhibit pH-independent photoluminescence (PL) emission, which is an advantageous property compared with the pH-dependent photoluminescence intensity variations, generally observed for the C-dots dispersed in aqueous solution. The synthesized C-dots and the nanocomposite films are characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infra-red spectroscopy (FTIR), ultraviolet (UV) − visible spectroscopy and photoluminescence spectroscopy (PL) techniques. The TEM image provides clear evidence for the formation of C-dots of almost uniform shape and average size of about 8 nm, homogeneously dispersed in aqueous medium. The strong anchoring of C-dots within the polymer matrix can be confirmed from the XRD results. The FTIR spectral studies conclusively establish the presence of oxygen functional groups on the surfaces of the C-dots. The photoluminescence (PL) emission spectra of the nanocomposite films are broad, covering most part of the visible region. The PL spectra do not show any luminescence intensity variations, when the pH of the medium is changed from 1 to 11. The pH-independent luminescence, shown by these films offers ample scope for using them as coatings for designing diagnostic and imaging tools in bio medical applications. The non-toxic nature of these nanocomposite films has been established on the basis of cytotoxicity studies.
Datum: 17.07.2017


An OFF–ON–OFF type fluorescent probe based on a naphthalene derivative for Al3+ and F− ions and its biological application

A novel fluorescent probe-based naphthalene Schiff, 1-(C2-glucosyl-ylimino-methyl)-naphthalene-2-ol (L) was synthesized by coupling d-glucosamine hydrochloride with 2-hydroxy-1-naphthaldehyde. It exhibited excellent selectivity and highly sensitivity for Al3+ in ethanol with a strong fluorescence response, while other common metal ions such as Pb2+, Mg2+, Cu2+, Co2+, Ni2+, Cd2+, Fe2+, Mn2+, Hg2+, Li+, Na+, K+, Fe3+, Cr3+, Zn2+, Ag+, Ba2+ and Ca2+ did not cause the same fluorescence response. The probe selectively bound Al3+ with a binding constant (Ka) of 5.748 × 103 M−1 and a lowest detection limit (LOD) of 4.08 nM. Moreover, the study found that the fluorescence of the L − Al3+ complex could be quenched after addition of F− in the same medium, while other anions, including Cl−, Br−, I−, NO2−, NO3−, ClO4−, CO32−, HCO3−, SO42−, HSO4−, CH3COO−, PO43−, HPO42−, S2− and S2O32− had nearly no influence on probe behaviour. Binding of the [L − Al3+] complex to a F− anion was established by different fluorescence titration studies, with a detection limit of 3.2 nM in ethanol. The fluorescent probe was also successfully applied in the imaging detection of Al3+ and F− in living cells.
Datum: 17.07.2017


Life prediction for a vacuum fluorescent display based on two improved models using the three-parameter Weibull right approximation method

To obtain precise life information for vacuum fluorescent displays (VFDs), luminance degradation data for VFDs were collected from a group of normal life tests. Instead of exponential function, the three-parameter Weibull right approximation method (TPWRAM) was applied to describe the luminance degradation path of optoelectronic products, and two improved models were established. One of these models calculated the average life by fitting average luminance degradation data, and the other model obtained VFD life by combining the approximation method with luminance degradation test data from each individual sample. The results indicated that the test design under normal working stress was appropriate, and the selection of censored test data was simple. The two models improved by TPWRAM both revealed the luminance decaying law for VFD, and the pseudo failure time was accurately extrapolated. It was further confirmed by comparing relative error that using the second model gave a more accurate prediction of VFD life. The improved models in this study can provide technical references for researchers and manufacturers in aspects of life prediction methodology for its development.
Datum: 07.07.2017


Enhanced green emissions of Er3+/Yb3+ co-doped Gd2(MoO4)3 by co-excited up-conversion processes

Improving the emission from rare earth ions doped materials is of great importance to broaden their application in bio-imaging, photovoltaics and temperature sensing. The green emissions of Gd2(MoO4)3:Er3+/Yb3+ powder upon co-excitation with 980 and 808 nm lasers were investigated in this paper. Distinct enhancement of green emissions was observed compared with single laser excitation. Based on the energy level structure of Er3+, the enhancement mechanism was discussed. Moreover, the result of temperature-dependent enhancement revealed that the enhancement factor reached its maximum (2.5) as the sample heated to 120°C, which is due to the competition of two major thermal effects acting in the co-excited up-conversion processes. In addition, the same enhancement of green emissions was also observed in Gd2(MoO4)3:Er3+ powder and NaYF4:Er3+/Yb3+ powder.
Datum: 06.07.2017


‘Turn-on’ fluorescent chemosensors based on naphthaldehyde-2-pyridinehydrazone compounds for the detection of zinc ion in water at neutral pH

A series of naphthaldehyde-2-pyridinehydrazone derivatives were discovered to display interesting ‘turn-on’ fluorescence response to Zn2+ in 99% water/DMSO (v/v) at pH 7.0. Mechanism study indicated that different substituent groups in the naphthaldehyde moiety exhibited significant influence on the detection of Zn2+. The electron rich group resulted in longer fluorescence wavelengths but smaller fluorescence enhancement for Zn2+. Among these compounds, 1 showed the highest fluorescence enhancement of 19-fold with the lowest detection limit of 0.17 μmol/L toward Zn2+. The corresponding linear range was at least from 0.6 to 6.0 μmol/L. Significantly, 1 showed an excellent selectivity toward Zn2+ over other metal ions including Cd2+.
Datum: 06.07.2017


Design and synthesis of the BODIPY–BSA complex for biological applications

A quinoxaline-functionalized styryl–BODIPY derivative (S1) was synthesized by microwave-assisted Knoevenagel condensation. It exhibited fluorescence enhancement upon micro-encapsulation into the hydrophobic cavity of bovine serum albumin (BSA). The S1–BSA complex was characterized systematically using ultraviolet (UV)–visible absorption, fluorescence emission, kinetics, circular dichroism and time-resolved lifetime measurements. The binding nature of BSA towards S1 was strong, and was found to be stable over a period of days. The studies showed that the S1–BSA complex could be used as a new biomaterial for fluorescence-based high-throughput assay for kinase enzymes.
Datum: 06.07.2017


 


Category: Current Chemistry Research

Last update: 04.01.2018.






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