Acclimatization and Establishment of Micropropagation Plants

Abstract

Plant propagation by tissue culture is known as micropropagation. Four sequential stages are known in micropropagation system are as under:

- Establishment

- Multiplication

- Pretransplant

- Transplant

The basic procedure for stage one is to establish the explant in culture, screen for contaminated explant and allow excised plant unit to stabilized to the culture environment. Any plant tissue or organ could be used as explant source, but it success depend upon the culture system, species being cultured, and removal of the contaminant from the explants .The stage one provide large percentage of explants free from surface pathogens. In establishment stage a sterile explant is produced and planted on sterile basal medium, which include, semisolid medium, agar, mineral salts with minor and major element, sucrose use as a source of energy, vitamin supplements, hormone, plant growth regulator, cytokinin, auxin and gibberellin. Usually 4-6 weeks required to complete stage one.

Establishment

This stage is used to establish as sterile explant in culture. The various factors effect on this stage are:

a) Choice of explant

b) Elimination of contaminant from the explant

c) Culture condition like ingredients, light, temperature

d) Choice of explant export

e) Environmental conditions

The appropriate explant use can come from knowledge of the plant species and their cultivars.